Caco-2-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > Caco-2-CAS9

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Caco-2-CAS9 Order

Caco-2-CAS9 cell line
Catalog#
YC-D003-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium(Caco-2)
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
Caco-2-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
Caco-2-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Caco-2 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Caco-2-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
Caco-2-CAS9 Cas9 19.79857635 3.43322181
Caco-2-CAS9 h-actin-B 16.36535454
Caco-2 Cas9 36.82312775 19.86952782
Caco-2 h-actin-B 16.95359993
Cutting Efficiency Validation
Caco-2-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the Caco-2-CAS9 stable cell pool which is electroporated by SDHB gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Caco-2		 STR Info (Cell bank)
Cell Line:CACO-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 1114111413.0
D7S820 11121112
D16S539 12121213
VWA 16181618
TH01 6666
AMEL XXXX
TPOX 911911
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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